Hotspot

ndhF

SSC · 111966-114197 · 2232 bp

Back to Anemone

Pi 0.0178

MarkerSeek score 60.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 144
pi: 0.0178
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATTTGGTGGTGGTGAACTCC	CTCACTCCGTTCTCCAACAG	50.0	55.0	58.0	57.9	2922-2966 bp	0.545455	68.0
ndhF_p2	ATTTGGTGGTGGTGAACTCC	AACAGGCTAGTTCACAACCG	50.0	50.0	58.0	58.1	2907-2951 bp	0.545455	67.6
ndhF_p3	TGGTGAACTCCCGCTTACTA	CTCACTCCGTTCTCCAACAG	50.0	55.0	58.1	57.9	2913-2957 bp	0.545455	67.5
ndhF_p4	GAACTCCCGCTTACTACTGC	CTCACTCCGTTCTCCAACAG	55.0	55.0	58.1	57.9	2909-2953 bp	0.545455	67.5
ndhF_p5	ATTTGGTGGTGGTGAACTCC	AAGCAACTCACTCCGTTCTC	50.0	50.0	58.0	57.8	2928-2972 bp	0.545455	67.3