Hotspot

ndhG-ndhI

SSC · 120649-121022 · 374 bp

Back to Anemone

Pi 0.0499

MarkerSeek score 81.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 374
variable_sites: 65
pi: 0.0499
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGTCCAGGCAAATCCAT	TTCAAGAACAATTACCAATTACT	45.0	26.1	57.4	52.3	419-535 bp	1.0	52.0
ndhG-ndhI_p2	ATTGGTCCAGGCAAATCCAT	GATTCAAGAACAATTACCAATTACT	45.0	28.0	57.4	54.1	421-537 bp	1.0	52.0
ndhG-ndhI_p3	ATTGGTCCAGGCAAATCCAT	ATTCAAGAACAATTACCAATTACT	45.0	25.0	57.4	52.9	420-536 bp	1.0	52.0
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	GATTCAAGAACAATTACCAATTAC	45.0	29.2	57.4	52.7	421-537 bp	1.0	52.0
ndhG-ndhI_p5	TCATGTATTGGTCCAGGCAA	TTCAAGAACAATTACCAATTACT	45.0	26.1	56.8	52.3	425-541 bp	1.0	52.0