Hotspot

ccsA-ndhD

SSC · 117157-117411 · 255 bp

Back to Anemone

Pi 0.0794
MarkerSeek score 85.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
255
variable_sites
59
pi
0.0794
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCTGCAATTGTGGCTTCT ATCCGGATTTCGTTCTCTCA 40.0 45.0 55.9 56.4 415-647 bp 1.0 62.1
ccsA-ndhD_p2 ATTCTGCAATTGTGGCTTCT TCCGGATTTCGTTCTCTCAT 40.0 45.0 55.9 56.4 414-646 bp 1.0 62.1
ccsA-ndhD_p3 TCAAATTCTGCAATTGTGGCT ATCCGGATTTCGTTCTCTCA 38.1 45.0 57.0 56.4 419-651 bp 0.909091 59.0
ccsA-ndhD_p4 TCAAATTCTGCAATTGTGGCT TCCGGATTTCGTTCTCTCAT 38.1 45.0 57.0 56.4 418-650 bp 0.909091 59.0
ccsA-ndhD_p5 TCAAATTCTGCAATTGTGGCT TCCGGATTTCGTTCTCTCATT 38.1 42.9 57.0 57.1 418-650 bp 0.909091 57.0