Hotspot

ndhF-rpl32

SSC · 114198-115856 · 1659 bp

Back to Anemone

Pi 0.0446
MarkerSeek score 77.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1659
variable_sites
224
pi
0.0446
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGGGGGACGAAAGGTATGA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 983-2212 bp 1.0 91.6
ndhF-rpl32_p2 AAGGGGGACGAAAGGTATGA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 984-2213 bp 1.0 91.6
ndhF-rpl32_p3 ACGAAAGGTATGATCCACGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 976-2205 bp 1.0 91.4
ndhF-rpl32_p4 CGAAAGGTATGATCCACGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 975-2204 bp 1.0 91.4
ndhF-rpl32_p5 AAGGGGGACGAAAGGTATGA GGAGGAGGCAATTCGCAATA 50.0 50.0 58.0 58.0 909-2138 bp 0.818182 84.2