Hotspot

rps12-clpP

LSC · 70805-70970 · 166 bp

Back to Anemone

Pi 0.0446

MarkerSeek score 78.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 20
pi: 0.0446
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	AGAGCGGGGGATTTTGTTAC	ATCGAAAATGCCGGGGATTT	50.0	45.0	57.9	58.2	243-266 bp	1.0	87.8
rps12-clpP_p2	GAGCGGGGGATTTTGTTACA	ATCGAAAATGCCGGGGATTT	50.0	45.0	58.2	58.2	242-265 bp	1.0	87.4
rps12-clpP_p3	AGAGCGGGGGATTTTGTTAC	GAAAATGCCGGGGATTTCAC	50.0	50.0	57.9	57.7	240-263 bp	1.0	87.0
rps12-clpP_p4	GAGCGGGGGATTTTGTTACA	GAAAATGCCGGGGATTTCAC	50.0	50.0	58.2	57.7	239-262 bp	1.0	86.6
rps12-clpP_p5	CACCCGAGTACATGTTCCTC	ATCGAAAATGCCGGGGATTT	55.0	45.0	57.7	58.2	283-306 bp	1.0	85.8