Hotspot

petL-petG

LSC · 66985-67159 · 175 bp

Back to Anemone

Pi 0.0362

MarkerSeek score 75.20

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 21
pi: 0.0362
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTCTGAGCAAGATACGAC	ACGAATAACCCAGCCAAAGT	50.0	45.0	57.1	57.4	262-292 bp	1.0	77.5
petL-petG_p2	TTGGTCTGAGCAAGATACGAC	ACGAATAACCCAGCCAAAGT	47.6	45.0	57.8	57.4	263-293 bp	1.0	75.2
petL-petG_p3	GGTCTGAGCAAGATACGACT	ACGAATAACCCAGCCAAAGT	50.0	45.0	56.8	57.4	261-291 bp	1.0	75.2
petL-petG_p4	TGGTCTGAGCAAGATACGAC	ACGCAGTTACGAATAACCCA	50.0	45.0	57.1	57.3	270-300 bp	0.909091	72.2
petL-petG_p5	TGGTCTGAGCAAGATACGAC	AGGCCTAACACGATTCCAAA	50.0	45.0	57.1	57.1	232-262 bp	0.909091	71.4