Hotspot

trnG-UCC-trnS-GCU

LSC · 46434-47606 · 1173 bp

Back to Anemone

Pi 0.124

MarkerSeek score 84.60

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 408
pi: 0.124
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	ACGAATCACACTTTTACCACT	TTATTCGTACCGAGGGTTCG	38.1	50.0	55.5	57.2	1114-1458 bp	1.0	56.9
trnG-UCC-trnS-GCU_p2	ACGAATCACACTTTTACCACT	TTCGAATCCCTCTCTTTCCG	38.1	50.0	55.5	57.0	1098-1442 bp	1.0	56.0
trnG-UCC-trnS-GCU_p3	AGAACGAATCACACTTTTACCAC	TTATTCGTACCGAGGGTTCG	39.1	50.0	57.3	57.2	1117-1461 bp	1.0	55.9
trnG-UCC-trnS-GCU_p4	GAACGAATCACACTTTTACCACT	TTATTCGTACCGAGGGTTCG	39.1	50.0	57.3	57.2	1116-1460 bp	1.0	55.9
trnG-UCC-trnS-GCU_p5	ACGAATCACACTTTTACCACT	GGGTTCGAATCCCTCTCTTT	38.1	50.0	55.5	56.9	1101-1445 bp	1.0	55.1