Hotspot

ndhF-trnL-UAG

SSC · 112699-113028 · 330 bp

Back to Anemonastrum

Pi 0.0579
MarkerSeek score 80.70
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
330
variable_sites
34
pi
0.0579
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TGATCCACGCATATTGATACA AGCGTGTCTACCGATTTCAC 38.1 50.0 54.9 58.0 383-404 bp 1.0 58.0
ndhF-trnL-UAG_p2 TGATCCACGCATATTGATACA CAGCGTGTCTACCGATTTCA 38.1 50.0 54.9 58.0 384-405 bp 1.0 57.9
ndhF-trnL-UAG_p3 TGATCCACGCATATTGATACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 54.9 57.9 393-414 bp 1.0 56.8
ndhF-trnL-UAG_p4 TGATCCACGCATATTGATACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 54.9 57.8 392-413 bp 1.0 56.2
ndhF-trnL-UAG_p5 TGATCCACGCATATTGATACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 54.9 57.8 391-412 bp 1.0 56.2