Hotspot

petA-psbJ

LSC · 62072-62490 · 419 bp

Back to Anemonastrum

Pi 0.0738

MarkerSeek score 80.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 57
pi: 0.0738
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGATTTGGTCAGGGAGATG	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	932-962 bp	1.0	87.8
petA-psbJ_p2	CAGCGTCGGGTATAGTAAGC	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1113-1143 bp	1.0	86.5
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1254-1284 bp	1.0	85.8
petA-psbJ_p4	TCCGATCGTATTTCTCCGGA	ACACAATCAAACCCGAACGA	50.0	45.0	58.0	58.0	1378-1408 bp	1.0	85.6
petA-psbJ_p5	TCCGATCGTATTTCTCCGGA	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1700-1730 bp	1.0	84.5