Hotspot

accD-psaI

LSC · 57865-58483 · 619 bp

Back to Anemonastrum

Pi 0.0511

MarkerSeek score 81.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 57
pi: 0.0511
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGGTTCTGTCCTTTGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	728-731 bp	1.0	89.9
accD-psaI_p2	TTCACGGGTTCTGTCCTTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	730-733 bp	1.0	89.9
accD-psaI_p3	CACGGGTTCTGTCCTTTGAA	GGAGTCCCGTCGAATCTAGA	50.0	55.0	58.0	58.0	784-787 bp	1.0	89.6
accD-psaI_p4	GGTGACTGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	942-945 bp	1.0	88.7
accD-psaI_p5	GGTGACTGCCAGTTTTGGTA	GGAGTCCCGTCGAATCTAGA	50.0	55.0	58.0	58.0	998-1001 bp	1.0	88.5