Hotspot

ndhC-trnV-UAC

LSC · 49548-50472 · 925 bp

Back to Anemonastrum

Pi 0.0833

MarkerSeek score 84.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 136
pi: 0.0833
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGCCAAAACCCCGGAAATTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.7	57.8	961-1102 bp	1.0	84.6
ndhC-trnV-UAC_p2	GGCCAAAACCCCGGAAATTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.7	57.5	960-1101 bp	1.0	81.8
ndhC-trnV-UAC_p3	GGCCAAAACCCCGGAAATTA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.7	58.7	949-1090 bp	1.0	80.7
ndhC-trnV-UAC_p4	GCCAAAACCCCGGAAATTAT	AGAAGGTCTACGGTTCGAGT	45.0	50.0	56.6	57.8	960-1101 bp	1.0	79.5
ndhC-trnV-UAC_p5	GGCCAAAACCCCGGAAATTA	AAGGTCTACGGTTCGAGTCC	50.0	55.0	58.7	58.8	959-1100 bp	1.0	79.5