Hotspot

trnF-GAA-ndhJ

LSC · 47322-47840 · 519 bp

Back to Anemonastrum

Pi 0.0652

MarkerSeek score 82.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 56
pi: 0.0652
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GCCCCAAACTTCTATGAAATCC	50.0	45.5	58.1	57.3	589-774 bp	1.0	68.6
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	GCCCCAAACTTCTATGAAATCC	50.0	45.5	57.5	57.3	592-777 bp	1.0	65.3
trnF-GAA-ndhJ_p3	AAGAGTCGGGATAGCTCAGT	GCCCCAAACTTCTATGAAATCC	50.0	45.5	57.6	57.3	632-817 bp	1.0	65.1
trnF-GAA-ndhJ_p4	AGAGTCGGGATAGCTCAGTT	GCCCCAAACTTCTATGAAATCC	50.0	45.5	57.6	57.3	631-816 bp	1.0	65.1
trnF-GAA-ndhJ_p5	TCGGGATAGCTCAGTTGGTA	GCCCCAAACTTCTATGAAATCC	50.0	45.5	57.6	57.3	627-812 bp	1.0	65.1