Hotspot

trnD-GUC-psbM

LSC · 23032-24203 · 1172 bp

Back to Anemonastrum

Pi 0.0617

MarkerSeek score 89.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1172
variable_sites: 128
pi: 0.0617
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AGTTCAATCGGTCAGAGCAC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.9	55.8	1271-1285 bp	1.0	73.5
trnD-GUC-psbM_p2	AGTTCAATCGGTCAGAGCAC	AGGAACTAGAATGAACAGTGCA	50.0	40.9	57.9	57.4	1278-1292 bp	1.0	70.3
trnD-GUC-psbM_p3	AGTTCAATCGGTCAGAGCAC	GGAACTAGAATGAACAGTGCAG	50.0	45.5	57.9	57.1	1277-1291 bp	1.0	67.8
trnD-GUC-psbM_p4	GTTCAATCGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	55.0	45.0	58.9	55.8	1270-1284 bp	1.0	66.8
trnD-GUC-psbM_p5	AGTTCAATCGGTCAGAGCAC	GGAACTAGAATGAACAGTGCA	50.0	42.9	57.9	55.9	1277-1291 bp	1.0	66.5