Hotspot

rps4-rps16

LSC · 645-1231 · 587 bp

Back to Anemonastrum

Pi 0.0907

MarkerSeek score 82.90

Primer available yes

Label rps4-rps16

Feature Metadata

label_name: rps4-rps16
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 95
pi: 0.0907
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-rps16_p1	CGCTCCGGAAAAAGATCTCA	TGGCGGGAACATTTGAAGAA	50.0	45.0	58.0	58.0	1248 bp	0.25	49.8
rps4-rps16_p2	ACGCTCCGGAAAAAGATCTC	TGGCGGGAACATTTGAAGAA	50.0	45.0	58.0	58.0	1249 bp	0.25	49.8
rps4-rps16_p3	CGCTCCGGAAAAAGATCTCA	GCGGGAACATTTGAAGAAGC	50.0	50.0	58.0	58.0	1246 bp	0.25	49.8
rps4-rps16_p4	ACGCTCCGGAAAAAGATCTC	GCGGGAACATTTGAAGAAGC	50.0	50.0	58.0	58.0	1247 bp	0.25	49.8
rps4-rps16_p5	CGCTCCGGAAAAAGATCTCA	ATGGGGAAACGTAGGGAAGA	50.0	50.0	58.0	58.0	1456 bp	0.25	49.6