Hotspot

ycf1

SSC · 120661-126105 · 5445 bp

Back to Androsace

Pi 0.0489

MarkerSeek score 77.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 1258
pi: 0.0489
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAATCGCGTTTTCTTGGTT	ATCACAGCCCCAAGTTCTTG	45.0	50.0	58.2	58.1	1128-1134 bp	0.2	49.3
ycf1_p2	CACCGTCCCATCTCAATTGA	ATCACAGCCCCAAGTTCTTG	50.0	50.0	57.6	58.1	1253-1259 bp	0.2	47.8
ycf1_p3	ATTCAAAGCACCGTCCCATC	ATCACAGCCCCAAGTTCTTG	50.0	50.0	58.5	58.1	1261-1267 bp	0.2	46.7
ycf1_p4	GATTCAAAGCACCGTCCCAT	ATCACAGCCCCAAGTTCTTG	50.0	50.0	58.5	58.1	1262-1268 bp	0.2	46.7
ycf1_p5	TGCTTCCGTATTGCTCTTGT	ATCACAGCCCCAAGTTCTTG	45.0	50.0	57.5	58.1	1198-1204 bp	0.166667	45.1