Hotspot

ndhF-rpl32

SSC · 109409-110093 · 685 bp

Back to Androsace

Pi 0.064
MarkerSeek score 77.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
685
variable_sites
166
pi
0.064
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.77
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAAGCCTTTTTGTCGCGTTT TTTTGACTGCCCAGTGGAAA 45.0 45.0 58.2 57.8 758-926 bp 0.133333 49.9
ndhF-rpl32_p2 GAAGCCTTTTTGTCGCGTTT TTTTTGACTGCCCAGTGGAA 45.0 45.0 58.2 57.8 759-927 bp 0.133333 49.8
ndhF-rpl32_p3 AAAAGCCCACATACGACGAA TTTTGACTGCCCAGTGGAAA 45.0 45.0 57.8 57.8 775-943 bp 0.133333 49.7
ndhF-rpl32_p4 AAAAGCCCACATACGACGAA TTTTTGACTGCCCAGTGGAA 45.0 45.0 57.8 57.8 776-944 bp 0.133333 49.7
ndhF-rpl32_p5 CCTTTTTGTCGCGTTTGGAA TTTTGACTGCCCAGTGGAAA 45.0 45.0 57.8 57.8 754-922 bp 0.133333 49.6