Hotspot

ndhC-trnV-UAC

LSC · 49948-51040 · 1093 bp

Back to Androsace

Pi 0.0584
MarkerSeek score 85.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1093
variable_sites
307
pi
0.0584
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.95
markerseek_score
85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACATCATATTCATAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 28.0 55.0 55.4 58.7 877-1181 bp 1.0 52.0
ndhC-trnV-UAC_p2 ACATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 28.0 50.0 55.4 57.8 889-1193 bp 0.966667 50.7
ndhC-trnV-UAC_p3 ACATCATATTCATAAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 28.0 55.0 55.4 57.5 888-1192 bp 0.966667 50.7
ndhC-trnV-UAC_p4 AACATCATATTCATAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 26.9 50.0 56.0 57.8 890-1194 bp 0.966667 50.7
ndhC-trnV-UAC_p5 ACATCATATTCATAAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 26.9 50.0 55.8 57.8 889-1193 bp 0.966667 50.7