Hotspot

rpoB-trnC-GCA

LSC · 26538-27694 · 1157 bp

Back to Androsace

Pi 0.0569

MarkerSeek score 83.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 308
pi: 0.0569
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	TGGCGCATAGTCAAAAAGGT	50.0	45.0	58.0	58.1	1961-2000 bp	0.2	51.2
rpoB-trnC-GCA_p2	GAAAATACCGGATCCCCACC	TGGCGCATAGTCAAAAAGGT	55.0	45.0	58.0	58.1	2168-2207 bp	0.2	50.9
rpoB-trnC-GCA_p3	CGACAAGCCCTGATCAATGA	GAATGAGTGGCGCATAGTCA	50.0	50.0	58.0	58.1	1552-1591 bp	0.166667	50.0
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	GAATGAGTGGCGCATAGTCA	50.0	50.0	58.0	58.1	1968-2007 bp	0.166667	49.5
rpoB-trnC-GCA_p5	GAAAATACCGGATCCCCACC	GAATGAGTGGCGCATAGTCA	55.0	50.0	58.0	58.1	2175-2214 bp	0.166667	49.3