Hotspot

trnS-GCU-trnG-UCC

LSC · 8257-8941 · 685 bp

Back to Androsace

Pi 0.081

MarkerSeek score 83.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 237
pi: 0.081
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	TACCACTAAACTATACCCGC	55.0	45.0	58.6	54.0	568-763 bp	1.0	54.8
trnS-GCU-trnG-UCC_p2	GATTCGAACCCTCGGTACAA	TACCACTAAACTATACCCGC	50.0	45.0	57.3	54.0	619-814 bp	1.0	54.6
trnS-GCU-trnG-UCC_p3	CTTTCGTCCACTCAGCCATC	TTACCACTAAACTATACCCGC	55.0	42.9	58.6	54.9	569-764 bp	1.0	53.7
trnS-GCU-trnG-UCC_p4	GATTCGAACCCTCGGTACAA	TTACCACTAAACTATACCCGC	50.0	42.9	57.3	54.9	620-815 bp	1.0	53.5
trnS-GCU-trnG-UCC_p5	CGGAAAGAGAGGGATTCGAA	TACCACTAAACTATACCCGC	50.0	45.0	57.0	54.0	631-826 bp	1.0	52.2