Hotspot

trnK-UUU-rps16

LSC · 4148-4863 · 716 bp

Back to Androsace

Pi 0.0839
MarkerSeek score 81.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
716
variable_sites
263
pi
0.0839
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCGTGGCCTTTCAAACGTTA AAAAGGGTATGTTGCTGCCA 45.0 45.0 58.0 58.0 1428-1534 bp 0.2 55.7
trnK-UUU-rps16_p2 CGTGGCCTTTCAAACGTTAC CCCAATGAGCCGTTTATCGA 50.0 50.0 58.0 58.1 951-1063 bp 0.166667 54.6
trnK-UUU-rps16_p3 CGTGGCCTTTCAAACGTTAC TCCCAATGAGCCGTTTATCG 50.0 50.0 58.0 58.1 952-1064 bp 0.166667 54.6
trnK-UUU-rps16_p4 CGTGGCCTTTCAAACGTTAC AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1427-1533 bp 0.166667 54.2
trnK-UUU-rps16_p5 TGGCCTTTCAAACGTTACCA AAAAGGGTATGTTGCTGCCA 45.0 45.0 57.9 58.0 1425-1531 bp 0.166667 54.0