Hotspot

trnH-GUG-psbA

LSC · 93-326 · 234 bp

Back to Androsace

Pi 0.0956

MarkerSeek score 80.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 86
pi: 0.0956
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCGAT	50.0	55.0	57.9	58.1	228-486 bp	1.0	90.2
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	CTGCGATTGAAGCTCCATCT	50.0	50.0	57.9	58.0	215-473 bp	0.966667	89.2
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	231-489 bp	0.966667	89.0
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	CTGCGATTGAAGCTCCATCT	50.0	50.0	58.2	58.0	216-474 bp	0.966667	89.0
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	232-490 bp	0.966667	88.8