Hotspot

rpl16

LSC · 77928-79430 · 1503 bp

Back to Andropogon

Pi 0.0116

MarkerSeek score 63.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1503
variable_sites: 129
pi: 0.0116
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	GGGTCGTCTAGCAGGAAAAG	55.0	55.0	57.9	58.0	1966-2039 bp	1.0	89.6
rpl16_p2	TTATACCGTCGTCGCCTTTG	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.1	58.0	2104-2177 bp	1.0	89.4
rpl16_p3	TCAGCAATAGTGTCCTTGCC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2409-2482 bp	0.973684	87.5
rpl16_p4	TTTCTAGAGGCATTTGGGGC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2038-2111 bp	0.921053	85.7
rpl16_p5	TCCTTGCCCATAAGACTCCA	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.0	58.0	2417-2470 bp	0.184211	49.1