Hotspot

ndhF-rpl32

SSC · 105673-106537 · 865 bp

Back to Andropogon

Pi 0.0166

MarkerSeek score 76.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 865
variable_sites: 112
pi: 0.0166
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	873-1001 bp	1.0	53.7
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	845-973 bp	1.0	53.4
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	846-974 bp	1.0	53.4
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	847-975 bp	1.0	53.4
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	848-976 bp	1.0	53.4