Hotspot

petL-petG

LSC · 63822-63988 · 167 bp

Back to Andropogon

Pi 0.0298

MarkerSeek score 54.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 23
pi: 0.0298
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	ACCCCAGCTCTATTTATTGGC	ACGCAGTCACGAATAATCCC	47.6	50.0	57.8	58.1	283-292 bp	1.0	77.7
petL-petG_p2	ACCCCAGCTCTATTTATTGGC	CAGTCACGAATAATCCCGCT	47.6	50.0	57.8	57.8	280-289 bp	1.0	76.5
petL-petG_p3	ACCCCAGCTCTATTTATTGGC	CGCCTGTATTGCAAATACGC	47.6	50.0	57.8	58.3	299-308 bp	1.0	75.7
petL-petG_p4	CCCCAGCTCTATTTATTGGCT	ACGCAGTCACGAATAATCCC	47.6	50.0	57.5	58.1	282-291 bp	1.0	75.4
petL-petG_p5	ACCCCAGCTCTATTTATTGGC	TGCAAATACGCAGTCACGAA	47.6	45.0	57.8	58.5	290-299 bp	1.0	74.0