Hotspot

trnD-GUC-psbM

LSC · 16829-17505 · 677 bp

Back to Andropogon

Pi 0.0134

MarkerSeek score 72.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 78
pi: 0.0134
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	831-1246 bp	1.0	63.2
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	797-1212 bp	1.0	61.8
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	832-1247 bp	1.0	61.1
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	832-1247 bp	1.0	59.9
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	792-1207 bp	1.0	59.8