Hotspot

trnG-UCC-trnT-GGU

LSC · 13919-15687 · 1769 bp

Back to Andropogon

Pi 0.0194
MarkerSeek score 72.40
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1769
variable_sites
249
pi
0.0194
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1340-2101 bp 1.0 52.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1336-2097 bp 1.0 52.0
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1337-2098 bp 1.0 52.0
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 38.1 45.0 55.5 56.7 1370-2131 bp 0.894737 48.7
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ACCACTAAGTGAAAAGCCCT 39.1 45.0 57.3 56.7 1372-2133 bp 0.894737 47.8