Hotspot

trnS-GCU-psbD

LSC · 8103-9066 · 964 bp

Back to Andropogon

Pi 0.00842

MarkerSeek score 65.90

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 964
variable_sites: 75
pi: 0.00842
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ACTCTACCAACGGCTATAGTCA	45.0	45.5	58.0	58.1	1005-1045 bp	0.973684	71.7
trnS-GCU-psbD_p2	GCTTTAGTCCACTCAGCCAT	ACTCTACCAACGGCTATAGTCA	50.0	45.5	57.6	58.1	991-1031 bp	0.973684	68.8
trnS-GCU-psbD_p3	AGTCCACTCAGCCATCTCTC	ACTCTACCAACGGCTATAGTCA	55.0	45.5	58.5	58.1	986-1026 bp	0.973684	68.0
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	CTCTACCAACGGCTATAGTCA	45.0	47.6	58.0	56.4	1004-1044 bp	0.973684	67.5
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	ACTCTACCAACGGCTATAGTC	45.0	47.6	58.0	56.4	1005-1045 bp	0.973684	67.5