Hotspot

rps18

LSC · 67067-67516 · 450 bp

Back to Anatherum

Pi 0.00376

MarkerSeek score 36.20

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 7
pi: 0.00376
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AAAGGAGGTTCATGGCCAAG	GACGACGAACAAAAATGCGT	50.0	45.0	58.1	58.0	1511-1584 bp	1.0	85.0
rps18_p2	GAAAGGAGGTTCATGGCCAA	GACGACGAACAAAAATGCGT	50.0	45.0	58.1	58.0	1512-1585 bp	1.0	84.9
rps18_p3	AAAGGAGGTTCATGGCCAAG	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.1	58.0	1371-1418 bp	0.96	83.3
rps18_p4	GAAAGGAGGTTCATGGCCAA	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.1	58.0	1372-1419 bp	0.96	83.3
rps18_p5	ATTGTCGCAAGCATACCACT	CGTTTGTGGATCACTCGGAT	45.0	50.0	57.9	58.0	1200-1247 bp	0.96	83.1