Hotspot

rpoC2

LSC · 26403-30842 · 4440 bp

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Pi 0.00241

MarkerSeek score 41.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4440
variable_sites: 42
pi: 0.00241
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CAGGAATTGTTAAATTTAGATATAGGA	ATATTCGCCCTCTCCCTCTC	25.9	55.0	53.7	58.1	113-230 bp	1.0	50.7
rpoC2_p2	TCAGGAATTGTTAAATTTAGATATAGG	ATATTCGCCCTCTCCCTCTC	25.9	55.0	53.7	58.1	114-231 bp	1.0	50.7
rpoC2_p3	AGGAATTGTTAAATTTAGATATAGGAC	ATATTCGCCCTCTCCCTCTC	25.9	55.0	53.7	58.1	112-229 bp	1.0	50.7
rpoC2_p4	CAGGAATTGTTAAATTTAGATATAGGA	TTCATATTCGCCCTCTCCCT	25.9	50.0	53.7	57.9	149-233 bp	0.96	44.9
rpoC2_p5	TCAGGAATTGTTAAATTTAGATATAGG	TTCATATTCGCCCTCTCCCT	25.9	50.0	53.7	57.9	150-234 bp	0.96	44.9