Hotspot

trnN-GUU-rps15

IRb · 102698-104292 · 1595 bp

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Pi 0.00111

MarkerSeek score 54.50

Primer available yes

Label trnN-GUU-rps15

Feature Metadata

label_name: trnN-GUU-rps15
feature_type: igs
region: IRb
length_bp: 1595
variable_sites: 11
pi: 0.00111
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rps15_p1	ACGACCAGTCAGTTAACAGC	AATGTGAAGCAAGTCTCCGT	50.0	45.0	57.8	57.4	1340-1739 bp	1.0	78.9
trnN-GUU-rps15_p2	AGGATTCGAACCTACGACCA	AATGTGAAGCAAGTCTCCGT	50.0	45.0	58.2	57.4	1353-1752 bp	1.0	78.8
trnN-GUU-rps15_p3	GGATTCGAACCTACGACCAG	AATGTGAAGCAAGTCTCCGT	55.0	45.0	57.8	57.4	1352-1751 bp	1.0	78.5
trnN-GUU-rps15_p4	ACCACTGAGCTACTGAGGAA	AATGTGAAGCAAGTCTCCGT	50.0	45.0	57.7	57.4	1310-1709 bp	1.0	77.6
trnN-GUU-rps15_p5	CCCCAAGTAGGATTCGAACC	AATGTGAAGCAAGTCTCCGT	55.0	45.0	57.7	57.4	1361-1760 bp	1.0	77.5