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trnD-GUC-psbM

LSC · 17223-18296 · 1074 bp

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Pi 0.00188

MarkerSeek score 54.80

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 15
pi: 0.00188
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AGAATAGGCACGCCATACAC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.8	57.9	853-1250 bp	0.96	56.6
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	817-1214 bp	1.0	56.5
trnD-GUC-psbM_p3	AGAATAGGCACGCCATACAC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.8	57.7	854-1251 bp	0.96	54.5
trnD-GUC-psbM_p4	AGAATAGGCACGCCATACAC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.8	55.5	854-1251 bp	0.96	53.2
trnD-GUC-psbM_p5	AGAATAGGCACGCCATACAC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.8	54.5	814-1211 bp	0.96	53.1