Hotspot

trnT-GGU-trnE-UUC

LSC · 16049-16581 · 533 bp

Back to Anatherum

Pi 0.0194

MarkerSeek score 58.20

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 25
pi: 0.0194
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.9	58.3	598-659 bp	1.0	76.0
trnT-GGU-trnE-UUC_p2	GAGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	47.6	50.0	57.9	57.7	577-638 bp	1.0	75.9
trnT-GGU-trnE-UUC_p3	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	597-658 bp	1.0	74.2
trnT-GGU-trnE-UUC_p4	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	560-621 bp	1.0	74.2
trnT-GGU-trnE-UUC_p5	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	576-637 bp	1.0	74.1