Hotspot

trnG-UCC-trnT-GGU

LSC · 14197-15976 · 1780 bp

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Pi 0.00548

MarkerSeek score 55.50

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1780
variable_sites: 49
pi: 0.00548
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCACTAAGTGAAAAGCCCT	38.1	45.0	55.5	56.7	1900-2133 bp	1.0	43.9
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TGAACCGATGACTTATGCCT	38.1	45.0	55.5	56.6	1870-2103 bp	1.0	43.7
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	GACTTCTGTCTTACCAAGGCA	38.1	47.6	55.5	57.6	1928-2161 bp	1.0	43.4
trnG-UCC-trnT-GGU_p4	ACGAATCACACTTTTACCACT	TGACTTCTGTCTTACCAAGGC	38.1	47.6	55.5	57.6	1929-2162 bp	1.0	43.4
trnG-UCC-trnT-GGU_p5	ACGAATCACACTTTTACCACT	CCGATGACTTATGCCTTACCA	38.1	47.6	55.5	57.5	1866-2099 bp	1.0	43.0