Hotspot

trnK-UUU-rps16

LSC · 3939-4494 · 556 bp

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Pi 0.00538
MarkerSeek score 52.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
556
variable_sites
11
pi
0.00538
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAAGAACA 50.0 47.6 58.3 58.2 677-723 bp 1.0 71.8
trnK-UUU-rps16_p2 CATAGCCGCACTTAAAAGCC GGTGCTCAACCTACAAGAACA 50.0 47.6 57.5 58.2 682-728 bp 1.0 70.2
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAAGAAC 50.0 50.0 58.3 56.4 677-723 bp 1.0 67.9
trnK-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC GGTGCTCAACCTACAAGAACA 50.0 47.6 57.2 58.2 676-722 bp 1.0 67.8
trnK-UUU-rps16_p5 TCTACCAAGAGTCTGGACGA GGTGCTCAACCTACAAGAACA 50.0 47.6 57.1 58.2 733-779 bp 1.0 66.8