Hotspot

rpl32-ndhF

SSC · 124970-126067 · 1098 bp

Back to Anaphalis

Pi 0.0111

MarkerSeek score 62.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1098
variable_sites: 71
pi: 0.0111
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1196-1303 bp	1.0	87.2
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1185-1292 bp	1.0	87.2
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1194-1301 bp	1.0	87.2
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	1195-1302 bp	1.0	87.1
rpl32-ndhF_p5	AACGCTGCCCAATATCCTTT	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1183-1290 bp	1.0	87.1