Hotspot

trnL-UAG-rpl32

SSC · 123999-124804 · 806 bp

Back to Anaphalis

Pi 0.0107

MarkerSeek score 65.10

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 806
variable_sites: 57
pi: 0.0107
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGCAGCGTTAAAAGCTTTGT	50.0	45.0	58.0	57.9	922-965 bp	1.0	87.8
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGCAGCGTTAAAAGCTTTGT	50.0	45.0	58.0	57.9	923-966 bp	1.0	87.8
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	AAAGGATATTGGGCAGCGTT	50.0	45.0	58.0	57.9	933-976 bp	1.0	87.7
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	AAAGGATATTGGGCAGCGTT	50.0	45.0	58.0	57.9	934-977 bp	1.0	87.6
trnL-UAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	GGCAGCGTTAAAAGCTTTGT	50.0	45.0	57.9	57.9	932-975 bp	1.0	86.8