Hotspot

ndhD-ccsA

SSC · 122582-122851 · 270 bp

Back to Anaphalis

Pi 0.0133

MarkerSeek score 65.90

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 270
variable_sites: 26
pi: 0.0133
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCTT	42.9	50.0	57.4	58.7	419-436 bp	1.0	70.1
ndhD-ccsA_p2	TCAGTTGACAAGGTTGAAGGT	TGGCGACTCTAGGCTTTCT	42.9	52.6	57.4	58.0	419-436 bp	1.0	67.8
ndhD-ccsA_p3	TCAGTTGACAAGGTTGAAGGT	GTGGCGACTCTAGGCTTTC	42.9	57.9	57.4	57.9	420-437 bp	1.0	67.6
ndhD-ccsA_p4	TCAGTTGACAAGGTTGAAGGT	CAATTGTGGCGACTCTAGGC	42.9	55.0	57.4	59.0	425-442 bp	0.978723	66.7
ndhD-ccsA_p5	TCAGTTGACAAGGTTGAAGGT	GCAATTGTGGCGACTCTAGG	42.9	55.0	57.4	59.0	426-443 bp	0.978723	66.7