Hotspot

accD-psaI

LSC · 59623-60285 · 663 bp

Back to Anaphalis

Pi 0.00503

MarkerSeek score 60.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 27
pi: 0.00503
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	868-905 bp	1.0	70.3
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	TTGCGATTGCCGGAAATACT	47.6	45.0	57.2	58.3	867-904 bp	1.0	70.2
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	TTGCGATTGCCGGAAATACT	45.0	45.0	56.2	58.3	869-906 bp	1.0	70.2
accD-psaI_p4	TCCAGAAGGGCTTATTCGATC	AGGCCTACTAAAGGGACCAA	47.6	50.0	57.2	57.6	848-885 bp	1.0	69.6
accD-psaI_p5	CCAGAAGGGCTTATTCGATCT	AGGCCTACTAAAGGGACCAA	47.6	50.0	57.2	57.6	847-884 bp	1.0	69.6