Hotspot

trnF-GAA-ndhJ

LSC · 48763-49443 · 681 bp

Back to Anaphalis

Pi 0.0105

MarkerSeek score 60.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 36
pi: 0.0105
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATAGCCGGGATAGCTCAGT	TGGATAGGATGGCCTTTACG	50.0	50.0	58.0	56.8	747-825 bp	1.0	77.8
trnF-GAA-ndhJ_p2	AATAGCCGGGATAGCTCAGT	GGATAGGATGGCCTTTACGT	50.0	50.0	58.0	56.8	746-824 bp	1.0	77.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	704-782 bp	1.0	77.7
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	703-781 bp	1.0	77.7
trnF-GAA-ndhJ_p5	AATAGCCGGGATAGCTCAGT	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.0	57.7	754-832 bp	1.0	77.2