Hotspot

psaA-ycf3

LSC · 42024-42810 · 787 bp

Back to Anaphalis

Pi 0.00979

MarkerSeek score 59.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 45
pi: 0.00979
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ACACATACAAAGAGACCCGC	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	880-934 bp	1.0	85.2
psaA-ycf3_p2	ACACATACAAAGAGACCCGC	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	882-936 bp	1.0	85.2
psaA-ycf3_p3	CATACAAAGAGACCCGCCAA	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	877-931 bp	1.0	85.1
psaA-ycf3_p4	CATACAAAGAGACCCGCCAA	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	879-933 bp	1.0	85.1
psaA-ycf3_p5	ATACAAAGAGACCCGCCAAC	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	876-930 bp	1.0	85.1