Hotspot

trnG-UCC-trnfM-CAU

LSC · 36558-36828 · 271 bp

Back to Anaphalis

Pi 0.0255

MarkerSeek score 67.90

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 32
pi: 0.0255
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	GAACGAAACAAAAAGCGGGT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.8	58.0	367-576 bp	1.0	88.0
trnG-UCC-trnfM-CAU_p2	GAACGAAACAAAAAGCGGGT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	57.8	58.0	403-612 bp	1.0	87.7
trnG-UCC-trnfM-CAU_p3	GAACGAAACAAAAAGCGGGT	GTCACGGGTTCAAATCCTGT	45.0	50.0	57.8	58.1	361-570 bp	1.0	87.6
trnG-UCC-trnfM-CAU_p4	GAACGAAACAAAAAGCGGGT	TCACGGGTTCAAATCCTGTC	45.0	50.0	57.8	57.8	360-569 bp	1.0	87.0
trnG-UCC-trnfM-CAU_p5	GAACGAAACAAAAAGCGGGT	TGAGGTCACGGGTTCAAATC	45.0	50.0	57.8	57.8	365-574 bp	1.0	86.9