Hotspot

trnT-GGU-psbD

LSC · 31556-32881 · 1326 bp

Back to Anaphalis

Pi 0.0134

MarkerSeek score 57.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1326
variable_sites: 95
pi: 0.0134
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CTTTGTCGAGGCACTTCTGT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1469-1915 bp	1.0	90.9
trnT-GGU-psbD_p2	CTTTGTCGAGGCACTTCTGT	ACACTGCGGCAGTTAAGAAA	50.0	45.0	58.1	58.0	1623-2069 bp	1.0	90.2
trnT-GGU-psbD_p3	CTTTGTCGAGGCACTTCTGT	ACAAAAGTCCACAGACCACC	50.0	50.0	58.1	57.9	1729-2175 bp	1.0	89.7
trnT-GGU-psbD_p4	CTTTGTCGAGGCACTTCTGT	TGGACCAGAGAATGCGATTG	50.0	50.0	58.1	58.0	1835-2281 bp	1.0	89.5
trnT-GGU-psbD_p5	CTTTGTCGAGGCACTTCTGT	CGGAATGTATTTGCGCCATC	50.0	50.0	58.1	58.0	2086-2532 bp	1.0	88.7