Hotspot

trnG-UCC-trnT-GGU

LSC · 31047-31492 · 446 bp

Back to Anaphalis

Pi 0.0364
MarkerSeek score 65.50
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
446
variable_sites
75
pi
0.0364
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.61
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ATTTGAACCGAGGGCTTACG 38.1 50.0 55.5 58.3 523-579 bp 0.361702 29.3
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT AGGGCTTACGCTTTACACTC 38.1 50.0 55.5 57.6 513-569 bp 0.361702 28.4
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT GAGGGCTTACGCTTTACACT 38.1 50.0 55.5 57.6 514-570 bp 0.361702 28.4
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT GGGCTTACGCTTTACACTCT 38.1 50.0 55.5 57.6 512-568 bp 0.361702 28.4
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT ATTTGAACCGAGGGCTTACG 39.1 50.0 57.3 58.3 525-581 bp 0.361702 28.3