Hotspot

psbM-trnD-GUC

LSC · 11021-11680 · 660 bp

Back to Anaphalis

Pi 0.00531

MarkerSeek score 57.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 26
pi: 0.00531
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.6	57.1	758-795 bp	1.0	51.9
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCTAGAGCACCG	40.0	50.0	54.5	57.1	752-789 bp	1.0	51.5
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.4	57.1	755-792 bp	1.0	50.6
psbM-trnD-GUC_p4	GGAACTAAAATGAACAGTGCAGT	CAATAGGCTAGAGCACCGC	39.1	57.9	57.6	58.1	756-793 bp	1.0	50.3
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCTAGAGCACCG	39.1	50.0	57.3	57.1	759-796 bp	1.0	49.9