Hotspot

petN-psbM

LSC · 10408-10915 · 508 bp

Back to Anaphalis

Pi 0.00738

MarkerSeek score 57.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 26
pi: 0.00738
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	663-700 bp	1.0	63.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	663-700 bp	1.0	62.2
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	50.0	40.0	57.1	55.6	664-701 bp	1.0	58.0
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.9	55.6	655-692 bp	1.0	58.0
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	47.6	40.0	58.0	55.6	649-686 bp	1.0	57.2