Hotspot

trnK-UUU-rps16

LSC · 4453-5295 · 843 bp

Back to Anaphalis

Pi 0.0146
MarkerSeek score 78.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
843
variable_sites
71
pi
0.0146
unique_haplotype_count
44
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 813-1110 bp 1.0 88.7
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTGAAAAAGGTGCTCAGCCT 55.0 45.0 57.9 57.9 812-1109 bp 1.0 88.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 752-1049 bp 1.0 88.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTGAAAAAGGTGCTCAGCCT 50.0 45.0 58.2 57.9 751-1048 bp 1.0 88.3
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC AAAAGGTGCTCAGCCTACAG 55.0 50.0 57.9 57.8 808-1105 bp 1.0 87.8