Hotspot

trnH-GUG-psbA

LSC · 79-481 · 403 bp

Back to Anaphalis

Pi 0.0335
MarkerSeek score 67.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
403
variable_sites
73
pi
0.0335
unique_haplotype_count
36
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AAGCTCCATCTACAAATGG 55.0 42.1 58.1 52.2 384-503 bp 1.0 52.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AAGCTCCATCTACAAATGG 50.0 42.1 57.9 52.2 398-517 bp 1.0 52.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AAGCTCCATCTACAAATGG 50.0 42.1 57.9 52.2 397-516 bp 1.0 52.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AAGCTCCATCTACAAATGG 50.0 42.1 57.9 52.2 399-518 bp 1.0 52.0
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC AAGCTCCATCTACAAATGG 50.0 42.1 57.5 52.2 413-532 bp 1.0 52.0