Hotspot

rpoC1

Genome · 22062-24818 · 2757 bp

Pi 0.00109

MarkerSeek score 56.80

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2757
variable_sites: 3
pi: 0.00109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AAAACCCGAATTCCAGGCAT	TTGCTCGTTCGAGAACTACG	45.0	50.0	58.1	58.0	2917-2927 bp	1.0	79.3
rpoC1_p2	AAAAACCCGAATTCCAGGCA	TTGCTCGTTCGAGAACTACG	45.0	50.0	58.3	58.0	2918-2928 bp	1.0	77.4
rpoC1_p3	GAAAAACCCGAATTCCAGGC	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.6	58.0	2919-2929 bp	1.0	76.8
rpoC1_p4	AAAACCCGAATTCCAGGCAT	GCTCGTTCGAGAACTACGAT	45.0	50.0	58.1	57.5	2915-2925 bp	1.0	75.9
rpoC1_p5	AAACCCGAATTCCAGGCATC	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.5	58.0	2916-2926 bp	1.0	75.6