Hotspot

psbC

Genome · 36183-37604 · 1422 bp

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Pi 0
MarkerSeek score 28.90
Primer available yes
Label psbC

Feature Metadata

label_name
psbC
feature_type
gene
region
Genome
length_bp
1422
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC_p1 TTTCCCAAGAAATCCGTGCA CCGAGCCAGAACAGAAAAGA 45.0 50.0 58.0 57.8 1582 bp 1.0 78.5
psbC_p2 GCAGCGGAAGATCCTGAATT CCGAGCCAGAACAGAAAAGA 50.0 50.0 58.3 57.8 1565 bp 1.0 76.0
psbC_p3 AGGTATTCGTGCTTGGATGG CCGAGCCAGAACAGAAAAGA 50.0 50.0 57.7 57.8 1509 bp 1.0 76.0
psbC_p4 GTTTCCCAAGAAATCCGTGC CCGAGCCAGAACAGAAAAGA 50.0 50.0 57.7 57.8 1583 bp 1.0 75.9
psbC_p5 TTCCCAAGAAATCCGTGCAG CCGAGCCAGAACAGAAAAGA 50.0 50.0 58.5 57.8 1581 bp 1.0 74.9