Hotspot

psbA

Genome · 71-1132 · 1062 bp

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Pi 0
MarkerSeek score 29.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
Genome
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TGGGTATTTTTCCTTCAGCA GTGCTTGGGAGTCCTTGAAA 40.0 50.0 54.8 58.0 1150 bp 0.5 34.4
psbA_p2 TGGGTATTTTTCCTTCAGCA AATACGTGTGCTTGGGAGTC 40.0 50.0 54.8 57.9 1157 bp 0.5 33.8
psbA_p3 TGGGTATTTTTCCTTCAGCAA GTGCTTGGGAGTCCTTGAAA 38.1 50.0 55.7 58.0 1150 bp 0.5 33.1
psbA_p4 TTGGGTATTTTTCCTTCAGCA GTGCTTGGGAGTCCTTGAAA 38.1 50.0 55.7 58.0 1151 bp 0.5 33.1
psbA_p5 TGGGTATTTTTCCTTCAGCAA AATACGTGTGCTTGGGAGTC 38.1 50.0 55.7 57.9 1157 bp 0.5 32.5